Device

Part:BBa_K352017

Designed by: Ozkan Is   Group: iGEM10_METU_Turkey   (2010-10-27)

pCooM RBS RFP Double terminator CooA coupled with placI+RBS

This part is formed by combination of pCooM Promoter coupled with RBS+RFP+Double terminator and CooA coupled with placI+RBS.


This component is used to measure high levels of carbon monoxide in the environment. Construction of this device was performed by utilizing strong CO sensitive promoter which is pCooM promoter from Rhodospirillum rubrum. This device is only active when there is CooA transcriptional activator and Carbon monoxide gas in the environment. When there is CO in the environment, the device produces red fluorescent protein in response.




Culturing CO Sensor E.coli with CO induction

Usage and Biology

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We have used 300 mL flasks with 80 mL LB culture. We have applied 80 Ml Carbon monoxide to some. The results were successful. Cultures induced with carbon monoxide successfully expressed red fluorescent protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 647
    Illegal AgeI site found at 759
    Illegal AgeI site found at 1617
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/reporter
//function/reporter/fluorescence
Parameters
controlspecific soret peak @ OD420
emission510 nm
excitation395 nm
familycooA is member of cAMP receptor protein (CRP) and fumavate nitrate reduction (FNR)
functionRFP reporter synthesized when carbon monoxide binding cooA protein exists
keggcooA: avn:Avin_47010
ligandsCO bound cooA
n/apCooM RBS RFP Double terminator CooA coupled with placI+RBS
outputRFP
uniprotcooA: P72322